cy3 affinipure donkey anti mouse igg  (Jackson Immuno)

Journal: Neurology® Neuroimmunology & Neuroinflammation

Article Title: Low Prevalence and Inconsistency of LRP4-IgG Detection in Suspected Myasthenia Gravis

doi: 10.1212/NXI.0000000000200554

Figure Lengend Snippet: Three protocols of CBA were performed: l-CBA (A–C), pf-CBA (D–F), and mf-CBA (G–I). 4′,6-diamidino-2-phenylindole (DAPI) (blue channel) shows nuclear staining, and green fluorescent protein (GFP) expression (green channel) indicates transfected cells. For each protocol, LRP4 antibody was visualized with Cy3 anti-mouse IgG (red fluorescence). CBA = cell-based assay; LRP4-IgG = lipoprotein receptor-related protein 4; mf-CBA = methanol-fixed CBA; pf-CBA = paraformaldehyde-fixed CBA.

Article Snippet: For its detection, Cy3 AffiniPure Donkey Anti-Mouse IgG (H + L) (Jackson Immuno Research #715-165-151) was used as the secondary antibody (1:1000).

Techniques: Staining, Expressing, Transfection, Fluorescence, Cell Based Assay

Journal: Neural Regeneration Research

Article Title: Overexpression of the inwardly rectifying potassium channel Kir4.1 or Kir4.1 Tyr 9 Asp in Müller cells exerts neuroprotective effects in an experimental glaucoma model

doi: 10.4103/NRR.NRR-D-24-00461

Figure Lengend Snippet: Effects of Kir4.1 overexpression and Kir4.1Tyr 9 Asp overexpression on GFAP expression in cultured Müller glial cells. (A, B) Representative immunoblots (A) and densitometric quantification (B) showing changes in GFAP expression in non-treated and DHPG-treated cultured Müller cells infected with lentiviruses overexpressing the eGFP control (LV-NC), Kir4.1, and Kir4.1 Tyr 9 Asp. All data are normalized to GADPH and then to Ctr. One-way analysis of variance (Tukey–Kramer multiple comparisons test) was performed (** P < 0.01, vs . Ctr group). (C) Double immunofluorescence staining showing changes in GFAP (Cy3, red) expression in non-treated (c1–c12) and DHPG-treated (c13–24) cultured Müller cells infected with lentiviruses overexpressing the eGFP Ctr (LV-NC) (c4–c6 and c16–c18), Kir4.1 (c7–c9 and c19–c21), and Kir4.1 Tyr 9 Asp (c10–c12 and c22–c24) mutation. Kir4.1 overexpression and Kir4.1 Tyr 9 Asp overexpression reduced the DHPG-induced increase in GFAP expression in Müller cells. GFP (indicated by Alexa488) and DAPI are shown in green and blue, respectively. Scale bars: 20 µm. Ctr: Control; DHPG: (S)-3,5-dihydroxyphenylglycine; eGFP: enhanced green fluorescent protein; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GFAP: glial fibrillary acidic protein; GFP: green fluorescent protein; LV-NC: eGFP control lentiviruses.

Article Snippet: The secondary antibodies used were donkey anti-mouse Cy3 (1:500, Cat# 715-165-151, RRID: AB_2315777) and donkey anti-rabbit 488 (1:500, Cat# 711-545-152, RRID: AB_2313584, Jackson ImmunoResearch Labs).

Techniques: Over Expression, Expressing, Cell Culture, Western Blot, Infection, Control, Double Immunofluorescence Staining, Mutagenesis

Journal: bioRxiv

Article Title: ATF4 and EcR interact to mediate both transcriptional activation and repression in the Drosophila fat body

doi: 10.64898/2026.03.10.710866

Figure Lengend Snippet: A) RT-qPCR analysis of indicated transcripts in isolated fat bodies from female animals expressing UAS transgenes encoding control ( LacZ RNAi ), ATF4 RNAi or EcR RNAi . UAS -transgene expression is driven by Dcg-GAL4 . Data represent average of four biological replicates and error bars represent standard error of mean. B) Representative confocal images showing 4E-BP intron -DsRed reporter expression (magenta) in female fat bodies also expressing indicated RNAi lines driven by Dcg-GAL4 . Nuclei are counterstained with DAPI (cyan). C) Quantification of nuclear DsRed in individual adipocytes from B . Data represent mean from at least 18 animals collected from three independent crosses. The dotted line indicates the average of the control ( LacZ RNAi ) sample. D) Relative Change in FRET after Acceptor (ATF4-GFP labeled with Cy5) photobleach upon excitation of the donor (EcR labeled with Cy3) in the nuclei of adipocytes from control ( w 1118 ) or ATF4-GFP expressing ( crc GFSTF /+ ) female animals. Data represent mean from at least 18 animals collected from three independent crosses. The dotted line indicates the average of the control ( w 1118 ) sample. Scale bar=50 μm. ****=p<0.00001; ***=p<0.0001; **=p<0.001; *=p<0.05. Only significant comparisons are shown. Statistical tests are described in the methods.

Article Snippet: Samples were incubated for 1 hour in the dark in donkey α-mouse Cy3 antibody (1:1000, Jackson ImmunoResearch #715-165-150) and donkey α-chicken Cy5 antibody (1:1000, Jackson ImmunoResearch #703-175-155) diluted in PBS.

Techniques: Quantitative RT-PCR, Isolation, Expressing, Control, Labeling

Journal: bioRxiv

Article Title: ATF4 and EcR interact to mediate both transcriptional activation and repression in the Drosophila fat body

doi: 10.64898/2026.03.10.710866

Figure Lengend Snippet: A) Representative confocal images showing 4E-BP intron -GFP reporter expression (magenta) in fat bodies also expressing LacZ RNAi , either ATF4 RNAi or usp RNAi , and both usp RNAi and ATF4 RNAi driven by Dcg-GAL4. Equal number of UAS-transgene cassettes were used by combining RNAi lines with either LacZ or LacZ RNAi . B) Quantification of nuclear GFP in individual adipocytes from A . Data represent mean from at least 18 animals collected from three independent crosses. The dotted line indicates the average of the control ( LacZ RNAi ) sample. C) Relative Change in FRET after Acceptor (Usp-GFP labeled with Cy5) photobleach upon excitation of the donor (EcR labeled with Cy3) in the nuclei of wandering third instar larval fat bodies where Dcg-GAL4 drives expression of control ( LacZ RNAi ) or ATF4 RNAi . Data represent mean from at least 18 animals collected from three independent crosses. The dotted line indicates the average of the control ( LacZ RNAi ) sample. Scale bar=50 μm. ****=p<0.00001; ***=p<0.0001; **=p<0.001; *=p<0.05. Only significant comparisons are shown. Statistical tests are described in the methods.

Article Snippet: Samples were incubated for 1 hour in the dark in donkey α-mouse Cy3 antibody (1:1000, Jackson ImmunoResearch #715-165-150) and donkey α-chicken Cy5 antibody (1:1000, Jackson ImmunoResearch #703-175-155) diluted in PBS.

Techniques: Expressing, Control, Labeling